Composite

Part:BBa_K3840312:Design

Designed by: Nefeli Zikou   Group: iGEM21_Greece_United   (2021-10-01)


Lamp2b Signal Peptide – CAP – GFP – Lamp2b – mir140


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 889
    Illegal XhoI site found at 571
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 82
    Illegal NgoMIV site found at 1468
    Illegal NgoMIV site found at 1957
    Illegal NgoMIV site found at 2023
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It went through codon optimization


Source

The sequence of this part was retrieved from the iGEM parts BBa_K3840000, BBa_K3840420, BBa_K3840040, BBa_K3840666, BBa_K3840069

References